Proteomics

The work in proteomics lab aims to identify and functionally characterize the proteins involved in different plant cellular mechanisms. Our research interests particularly focus on the systems which are involved in the regulation of cell redox homeostasis such as NADPH-dependent thioredoxin system and the proteins involved in chelation of metals such as metallothionein.
Since many gene products of biological interest are naturally produced in minute quantities, making it difficult to study them. To circumvent this problem, one solution is to produce recombinant form of these proteins. Therefore we use genetic engineering tools for isolation and cloning of genes encoding our proteins of interest. The recombinant proteins are heterologously expressed in prokaryotic or eukaryotic hosts. After protein purification, functional analysis of proteins is performed using protein chemistry and enzyme kinetics techniques along with protein engineering tools such as site-directed mutagenesis.

 

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